Composite

Part:BBa_K3814074

Designed by: Simon Tang   Group: iGEM21_Sydney_Australia   (2021-10-01)


Cluster 6

We aim to produce naturally transformable (NT) E. coli by inserting the NT genes of another species into it. We have chosen 23 genes from Acinetobacter baylyi and have planned to insert them into the fliK gene in the E. coli.

To do this, we have devised a novel recombineering strategy that allows for homologous recombination to insert large amounts of DNA sequentially into the chromosome. Thus, rather than inserting each of the 23 genes individually, we can transform them in clusters of genes.

We determined these clusters by assessing the biological function of each gene and through k-means clustering, and one such is shown here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1954
    Illegal NheI site found at 1977
    Illegal NheI site found at 2169
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2315
    Illegal BamHI site found at 3217
    Illegal XhoI site found at 75
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2341
    Illegal NgoMIV site found at 2709
    Illegal NgoMIV site found at 2869
  • 1000
    COMPATIBLE WITH RFC[1000]


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